Amino acid status and nitrogen forms of rumen contents in vitro when phytogenic components are added to the reaction medium

Federal Research Centre of Biological Systems and Agrotechnologies of the Russian Academy of Sciences, Orenburg, Russia

^* Corresponding author:daniilshoshin@mail.ru

Abstract

Phytobiotics are increasingly used in animal husbandry as an alternative to antibiotic drugs. However, before large-scale introduction of such substances into feeding practice, it is necessary to conduct a detailed analysis of their effects on physiological processes, particularly in polygastric animals where most energy is synthesized by the rumen microflora. This study aimed to investigate the indicators of nitrogen metabolism and amino acid composition of rumen fluid when quercetin, 7- hydroxycoumarin, vanillin, and trans-cinnamaldehyde were introduced into the in vitro reaction medium. The results showed that vanillin at a concentration of 49.00×10^-5 mol/l promoted the maximum increase in the level of aliphatic, aromatic, and oxymonocarboxylic amino acids, while quercetin and 7-hydroxycoumarin at 98.0×10^-5 and 24.50×10^-5 mol/l, respectively, best stimulated the accumulation of methionine. Additionally, vanillin at a dose of 24.50×10^-5 mol/l had a beneficial effect on the level of positively charged amino acids. The lowest values were found in the experiment with trans-cinnamaldehyde. These findings suggest that the presented phytobiotics, upon further consideration, can be used to regulate and manage the amino acid status of the rumen of ruminants.